Korean native goats were experimentally infected with bovine leukemia virus(BLV), and antibody responses and BLV in leucocytes were investigated by agar-gel immunodifusion test(AGID), complement dependent antibody cytotoxicity(CDAC), complement
fixation
test(CF) and virus neutralization test(VN) using syncytium inhibition assay.
AGID antibody titers of Group I inoculated with FLK-BLV cells were 2 to 4 at I month postinoculation(p.i.), and persisted for 12 months p.i. showing maximum titer of 16. Group II inoculated with the peripheral leucocytes from BLV-infected cattle
showed
the lower AGID antibody titer than Group I. Cytotoxicity of rabbit complement and normal goat serum against, FLK-BLV cells was investigated. Four out of 6 rabbit complements were revealed cytotoxicity index under 5.0%. Range of cytotoxic index of
17
normal goat sera were 11.8±28.6 to 28.6±9.7. CDAC antibody titers of Group I were 8 to 16 at 1 month p.i. and then increased gradually showing maximum titer of 128 at 6 months p.i., At 12 months p.i. CDAC titer were 32 to 128. Group II chowed
relatively lower CDAC titer than Group I. CF antibody titers of Group I were 4 to 8 at 1 months p.i., and then increased up to 32 to 128 at 8 to 10 months p.i., Group II showed the similai patterns of CF titers as Group I but No 3 revealed
particularly
lower CF titer throughout the experiments. In syncytium assay for detection of BLV in the peripheral lymphocytes. all six heads of goats in Group I and Group II revealed the positive SA through 12 months showing the maximum SA values of 148. The
SA
values tended to decrease as the observation time passed, VN antibody titers of Group I were 16 to 32 at 1 month p.i., and then increased gradually by the maximum titer of 128. Through 12 month experiment, Group II manifested the lower VN
antibody
titer
than Group I. Throughout 12 months period, the control groups that are Group III and Group IV, showed no antibody responses in the above tests.
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